Contact an Account ManagerDetermination of sulphonated azo dyestuffs and their bacterial metabolites by HPLC
Summary
Type: HPLC
Application #: 101610
Catalog#: 720018.46 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4.6x250mm
Detection: UV 220nm
Conditions: eluent A: 100mM K phosphate buffer, pH 6.7,
eluent B: methanol - 10mM K phosphate,
pH 6.7 (7:3 v/v),
10min eluent A, then linear gradient from 0 to 100% B in 30min,
flow rate 0.7ml/min
Authors: Grossenbacher H
Source: J Chromatogr 360 (1986) 219 - 223
Details
Legend: Chromatogram of a mixture of sulphonates,
I. sulphanilic acid,
II. p-hydroxybenzene sulphonic acid,
III. metanilic acid,
IV. orthanilic acid,
V. sulphanilamide,
VI. laurent acid,
VII. gamma a